| 标题 |
MiR432* regulate the replication of coxsackievirus A16 in rhabdomyosarcoma cells
MiR432*调节柯萨奇病毒A16在横纹肌肉瘤细胞中的复制
相关领域
生物
小RNA
病毒复制
病毒
转染
病毒学
基因
报告基因
非翻译区
载体（分子生物学）
基因表达
遗传学
信使核糖核酸
重组DNA
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| 网址 |
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AI链接 nih.gov |
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| 其它 |
Objective:MicroRNAs (miRNAs) play an important role in infection and replication of virus in host cells. In this study, we examined miRNAs' effects on the replication of Coxsackievirus A16 (CA16) in rhabdomyosarcoma cells. Methods:We constructed target gene of miRNAs screening system. We used 3'untranslated region (UTR) dual luciferase reporter analysis to identify putative miRNA targets in the CA16 virus genome. First, 12 segments of CA16 virus genome were inserted to the pMIR vector and the luciferase expression were assayed to identify the target gene of putative miRNA. The reporter gene expression of the cells transfected with the vector containing 5'-UTR was significantly downregulated. Then, using online analysis programs we screened the miRNAs that may target to 5'-UTR. Furthermore, Western blot and real-time PCR test were used to study the effect of miRNAs on viral replication. |
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