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[求助补充材料]
 Droplet-microarray on superhydrophobic–superhydrophilic patterns for high-throughput live cell screenings 相关领域
超亲水性
吞吐量
微阵列
化学
纳米技术
计算机科学
材料科学
润湿
复合材料
生物化学
基因表达
电信
基因
无线
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Cell culturing on Droplet-Microarray To evaluate the DMA platform for cell-based screening we optimized culturing conditions in individual droplets using three common cell lines: a human cervical cancer cell line (HeLa), human embryonic kidney cell line (HEK293), and human lung adenocarcinoma epithelial cell line (A549). To prepare the DMA slide to culture cells, we first immersed it in DMEM medium containing 1% FBS, followed by coating SL spots with 2.2% wt. gelatin. Droplet microarray was formed and the array was placed in a cell culture incubator in a humidified double Petri dish to prevent evaporation. All three cell lines cultured in individual droplets of different sizes for 24 hours revealed spread morphology similar to that of cells cultured in microplates (Fig. 3) and viability of cells in droplets was 97-100% as proven by live/dead staining (Fig. S1e). |
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