标题 |
Analysis of clonogenic growth in vitro
相关领域
体外
克隆形成试验
协议(科学)
计算生物学
模块化设计
计算机科学
生物
细胞生物学
体内
细胞培养
化学
生物化学
生物技术
遗传学
医学
病理
替代医学
操作系统
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摘要 The clonogenic assay measures the capacity of single cells to form colonies in vitro. It is widely used to identify and quantify self-renewing mammalian cells derived from in vitro cultures as well as from ex vivo tissue preparations of different origins. Varying research questions and the heterogeneous growth requirements of individual cell model systems led to the development of several assay principles and formats that differ with regard to their conceptual setup, 2D or 3D culture conditions, optional cytotoxic treatments and subsequent mathematical analysis. |
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