| 标题 |
 Quantitative analyses of nitrogen cycling genes in soils 土壤氮循环基因的定量分析
相关领域
自行车
固氮
氮气循环
土壤水分
生物
氮气
丰度（生态学）
基因
相对物种丰度
微生物生态学
植物
环境化学
生态学
化学
遗传学
细菌
有机化学
历史
考古
|
| 网址 | |
| DOI |
10.1016/i.pedobi.2005.05.005
doi
提醒：求助人提供的doi与AI识别不一致
10.1016/j.pedobi.2005.05.005
Doi
|
| 其它 |
期刊：Pedobiologia [Elsevier] 日期：2005-08-18 卷期号：49 (6): 665-672 被引量：113 DOI：10.1016/j.pedobi.2005.05.005 Soil nitrogen cycling is accomplished by a diverse microbial community using several nitrogen functional genes (NFGs). The relationship between the composition of these communities and nitrogen cycling processes is poorly understood. We developed techniques to quantify several important NFGs nitrogen transformations including fixation (nifH), ammonia oxidation (amoA), nitrogen reduction (nirS and nirK), and nitrous oxide reduction (nosZ) using quantitative real-time PCR with SybrGreen. Standard curves with known gene copies of each target molecule were developed using genomic DNA extracted from pure cultures. Primers from previously published studies were successfully used to amplify and quantify each NFGs in DNA extracted from two forest soils. Some soil samples inhibited PCR amplification of positive control DNA, |
| 求助人 | |
| 下载 | 该求助完结已超 24 小时，文件已从服务器自动删除，无法下载。 |
|
温馨提示：该文献已被科研通 学术中心 收录，前往查看
科研通『学术中心』是文献索引库，收集文献的基本信息（如标题、摘要、期刊、作者、被引量等），不提供下载功能。如需下载文献全文，请通过文献求助获取。
|
不配.
薰硝壤
Singularity
Leonardi
paparazzi221
可靠的书桃
一一
完美的天空
pcr163
HEIKU
寻道图强
咖啡豆
陈军
yufanhui
tuanheqi
pluto
8R60d8
sissiarno
坚强亦丝
Yziii
皖公网安备34019202002308
