| 标题 |
[Alveolar macrophages in rats with chronic obstructive pulmonary disease (COPD) promotes proliferation, mucin and inflammatory factors secretion of airway epithelial cells and its mechanism]
慢性阻塞性肺疾病（COPD）大鼠肺泡巨噬细胞促进气道上皮细胞增殖、粘蛋白和炎性因子分泌及其机制
相关领域
支气管肺泡灌洗
粘蛋白
免疫印迹
慢性阻塞性肺病
分泌物
化学
微泡
分子生物学
病理
医学
生物
肺
小RNA
内科学
生物化学
基因
|
| 网址 |
求助人暂未提供
AI链接 nih.gov |
| DOI |
暂未提供，该求助的时间将会延长，查看原因？
|
| 其它 | Objective To explore how alveolar macrophages from chronic obstructive pulmonary disease (COPD)-model rats affect proliferation and secretion of 16HBE human bronchial epithelial cells investigate the associated mechanism. Methods Alveolar were extracted COPD induced by cigarette smoke exposure LPS instillation through bronchoalveolar lavage, then co-cultured with in vitro. Exosomes exosome isolation kit. The differentially expressed miRNA exosomes derived group control was detected PCR. miR-380 overexpressed mimic while expression cystic fibrosis transmembrane transduction regulator (CFTR) knocked down siRNA cells. CCK-8 assay. migration ability evaluated TranswellTM levels mucins (MUC5AC, MUC5B, MUC2) CFTR Western blot analysis. TNF-α IL-6 supernatant ELISA. Results enhanced production as well increased macrophages. significantly elevated Both overexpression inhibition decreased CFTR, resulting MUC5AC, MUC2 TNF-α, IL-6. |
| 求助人 | |
| 下载 | 暂无链接，等待应助者上传 |
|
温馨提示：该文献已被科研通 学术中心 收录，前往查看
科研通『学术中心』是文献索引库，收集文献的基本信息（如标题、摘要、期刊、作者、被引量等），不提供下载功能。如需下载文献全文，请通过文献求助获取。
|
迷人秋烟
XD
QOP
liuyanjun
小透明
VDC
迟大猫
123
顽强的小刘
Liu_letters
xuelang
MchemG
三叔
安静的远山
clairevox
搜集达人
重生之我来找文献
ukz37752
GrindSeason
YifanWang
皖公网安备34019202002308
