Lvov YM, Decher G, Mohwald H. Assembly, structural characterization, and thermal behavior of layer-by-layer deposited ultrathin films of poly(vinyl sulfate) and poly(allylamine). Langmuir. 1993 Nov;9(11):481–7. doi: 10.1021/la00034a026. PMID: 22132590.
Arancibia-Bulnes CA, Suazo-Dávila D, Soto-Arriaza MA, Zúñiga RA, Shene C. Quaternization degree of chitosan influences the structure and the release profiles of drug loaded in hydrogels. Carbohydr Polym. 2015 Mar 6;117:728–36. doi: 10.1016/j.carbpol.2014.10.036. PMID: 25498627.
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具体来说，通过使用1H或13C NMR谱图，可以观察到季铵化壳聚糖分子中不同原子核的峰。其中，季铵化基团上的原子核峰通常与非取代的壳聚糖基团上的峰有所不同。因此，通过比较这些峰的强度，可以确定取代度。
文献推荐：
Lvov YM, Decher G, Mohwald H. Assembly, structural characterization, and thermal behavior of layer-by-layer deposited ultrathin films of poly(vinyl sulfate) and poly(allylamine). Langmuir. 1993 Nov;9(11):481–7. doi: 10.1021/la00034a026. PMID: 22132590.
Arancibia-Bulnes CA, Suazo-Dávila D, Soto-Arriaza MA, Zúñiga RA, Shene C. Quaternization degree of chitosan influences the structure and the release profiles of drug loaded in hydrogels. Carbohydr Polym. 2015 Mar 6;117:728–36. doi: 10.1016/j.carbpol.2014.10.036. PMID: 25498627.