Spiking should be carried out at the beginning of the whole analytical process. The target analyte should be added at the undiluted samples while authors should also explain the choice of the spiked concentrations of the analyte used in the study. Provide correct references using an original source of information with the experimental evidence (e.g., results of clinical studies) or normative documentation (e.g., from World Health Organization, from European Commission).
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(2025-6-4)
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