Carbon quantum dot with co-doped nitrogen and phosphorus for tazettine ratiometric fluorescent sensing

An overdose of tazettine (TZ) has toxic side effects, which makes it extremely important to rapidly and subtly determine the TZ. In this study, double-emitting, nitrogen-phosphorus co-doped carbon quantum dots (N,P-CQDs) were prepared using cis-butenedioic acid, phosphoric acid, and p-phenylenediamine. An effective inner filter effect (IFE) can occur between TZ and N,P-CQDs, resulting in their fluorescence quench. Therefore, a ratio fluorescent probe was constructed for detecting the TZ was constructed. After optimizing the experimental conditions, the quenching efficiency showed a strong linear connection with the TZ concentration in the range of 0.01-30 µmol/L, with the detection limit of 0.002 µmol/L. This method could be satisfactorily applied to detect TZ in mouse plasma samples.