Development of serum-free media

Abstract The removal of serum or other undefined components from cell culture medium involves a multifaceted approach. This is mainly due to the interactive nature of cell culture systems. Cell culture systems require various components: basal nutrients, a set of supplements that convey information, such as growth factors, mitogens, hormones, and cytokines, and for anchorage-dependent cells, a substrate. In designing any cell culture system attention must be paid to all these components and their interactions.