猴痘
生物
系统发育树
爆发
正痘病毒
基因组
病毒学
遗传学
进化生物学
遗传多样性
全基因组测序
新型病毒
基因
医学
牛痘
重组DNA
人口
环境卫生
作者
Katarina Resman Rus,Samo Zakotnik,Martin Sagadin,Nataša Knap,Alen Suljič,Tomaž Mark Zorec,Maja Mastnak,Miroslav Petrovec,Mario Poljak,Miša Korva,Tatjana Avšič-Županc
出处
期刊:Acta dermatovenerologica Alpina, Pannonica et Adriatica (Tiskana izd.)
[Association of Slovenian Dermatovenerologists]
日期:2023-01-01
卷期号:32 (3)
标识
DOI:10.15570/actaapa.2023.21
摘要
Monkeypox virus (MPXV), typically endemic in West and Central Africa, has raised global concern due to the recent outbreak in several non-endemic countries with human-to-human transmission. Here we present a comprehensive analysis of MPXV genomes from Slovenia.Two real-time polymerase chain reaction (RT-PCR) assays for Orthopoxvirus (OPV) and MPXV genes were used for laboratory confirmation of mpox. Complete MPXV genomic sequences were obtained using nanopore long reads and Illumina technology. Phylogenetic analyses compared the Slovenian MPXV sequences with the global sequences.A total of 49 laboratory-confirmed mpox cases were diagnosed in Slovenia in 2022, mainly affecting males under 40. In 48 cases, a complete genome sequence was obtained and phylogenetic analysis revealed five distinct lineages (B.1, B.1.14, B.1.2, B.1.3, and A.2.1), with B.1 and B.1.3 dominating, suggesting multiple introductions into Slovenia. Genome analysis revealed significant divergence from the reference MPXV-M5312_HM12_Rivers.The genetic diversity observed in the Slovenian MPXV sequences sheds light on the complex dynamics of the 2022 mpox outbreak and highlights the need for further research to understand the impact of mutations on MPXV functional characteristics and their role in the evolution and diversification of current lineages.
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