Bioactive phytochemicals from the tubers of Bletilla striata Rchb.f

AbstractThirty-four phytochemicals were isolated from dry tubers of Bletilla striata Rchb.f. The compounds were classified as bibenzyls 1–14, dihydrophenanthrenes 15, 17, 20, 21, phenanthrenes 16, 18, 19, simple benzoids 22–24, a fatty acid 25, glucosyloxybenzyl 2-isobutylmalates 26–32, and glucosyloxybenzyl cinnamates 33, 34. Compounds 1–4, 7, 8, 11, 12, and 16 inhibited melanogenesis (17.96–55.27%) induced by α-MSH in B16F10 cells at 10–40 μM. However, compounds 9, 10, 17, 18, and 21 exhibited significant cytotoxicity against melanomas, with IC50 values of 12–34 μM. Additionally, compounds 15, 17, 19, 20, 23, 31, and 33 reduced the ROS generation induced by H2O2 in HaCaT cells at 6.25–100 μM. In particular, compounds 15, 19, and 20 strongly inhibited ROS generation, with IC50 values of 2.15–9.48 μM. Consequently, compounds 1–4, 7–12, and 15–21 may be the strongest leads to follow in B. striata extract for further research on the skin disorders, hyperpigmentation, melanoma, and ageing.Keywords: Bletilla striata Rchb.f.Orchidaceaephytochemical analysisanti-ROS generationanti-melanogenesis AcknowledgmentsWe would like to thank Uni-edit (www.uni-edit.net) for editing and proofreading this manuscript. Mass spectrometric analyses were performed by the Proteomics Research Core Laboratory, Office of Research & Development at CMU.Disclosure statementThe authors have no potential conflicts of interest to report.Additional informationFundingThis study was supported by grants from the Ministry of Science and Technology (MOST 105-2320-B-039-047-MY3; MOST 108-2320-B-039-035-) and China Medical University (CMU109-S-63), Taiwan.