Functions and mechanisms of sponge iron-mediated multiple metabolic processes in anaerobic ammonium oxidation

Sponge iron (SI) is a promising material for nitrogen removal from wastewater. This study reveals the potential functions and mechanisms of SI-mediated multiple metabolic processes in the nitrogen removal of Anammox. The results showed that although the SI application prolonged the start-up time of the reactor, achieved efficient and stable nitrogen removal after a successful start-up. The total nitrogen removal efficiency of the SI-Anammox system (92.62%) was 13.30% higher than that of R0 without SI (79.32%). The increase in nitrogen removal performance was accompanied by an increase in SAA and EPS content. Further microbial analysis showed significant enrichment of functional microorganisms, such as Candidatus_Brocadia, Nitrosomonas, Ellin6067, and Nitrospira. Multi-omics evidence suggests that efficient nitrogen removal is ultimately attributable to the enhancement of the specific key Fe- and N-functional genes in Anammox.