热稳定性
酶动力学
脱氢酶
化学
异源的
生物化学
羟类固醇脱氢酶
羟类固醇
酶分析
辅因子
异源表达
羟类固醇脱氢酶
重组DNA
酶
活动站点
基因
作者
Deshuai Lou,Yangyang Cao,Hongtao Duan,Jun Tan,B. Li,Yuanjun Zhou,Dong Wang
出处
期刊:Protein and Peptide Letters
[Bentham Science]
日期:2024-02-01
卷期号:31 (2): 153-160
标识
DOI:10.2174/0109298665279004231229100320
摘要
7α-Hydroxysteroid dehydrogenase (7α-HSDH) plays a pivotal role in vivo in the biotransformation of secondary bile acids and has great potential in industrial biosynthesis due to its broad substrate specificity. In this study, we expressed and characterized a novel thermostable 7α-HSDH (named Sa 7α-HSDH).
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