红曲霉
紫色红曲霉
分泌物
细胞壁
变位酶
生物化学
生物
颜料
菌丝体
细胞外
微生物学
细胞生物学
化学
发酵
基因
植物
有机化学
作者
Xufeng Wang,Li Li,Chengfang Ding,Zhenjing Li,Wentao Ding,Huanhuan Liu,Nifei Wang,Changlu Wang,Qingbin Guo
标识
DOI:10.1016/j.ijbiomac.2024.129369
摘要
The impact of the cell wall structure of Monascus purpureus M9 on the secretion of extracellular monascus pigments (exMPs) was investigated. To modify the cell wall structure, UDP-galactopyranose mutase (GlfA) was knocked out using Agrobacterium-mediated transformation method, leading to a significant reduction in the Galf-based polysaccharide within the cell wall. Changes in mycelium morphology, sporogenesis, and the expression of relevant genes in M9 were also observed following the mutation. Regarding MPs secretion, a notable increase was observed in six types of exMPs (R1, R2, Y1, Y2, O1 and O2). Specifically, these exMPs exhibited enhancement of 1.33, 1.59, 0.8, 2.45, 2.89 and 4.03 times, respectively, compared to the wild-type strain. These findings suggest that the alteration of the cell wall structure could selectively influence the secretion of MPs in M9. The underlying mechanisms were also discussed. This research contributes new insights into the regulation of the synthesis and secretion of MPs in Monascus spp..
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