Arsenic Uptake and Metabolism in Mycorrhizal As-Hyperaccumulator Pteris vittata: Symbiotic P Transporters and As Reductases

Arbuscular mycorrhiza (AM) often protect host plants from As accumulation under arsenic stress; however, the opposite is true for the As-hyperaccumulator Pteris vittata. With non-hyperaccumulator Pteris ensiformis as a comparison, the AM colonization, P and As uptake, and genes associated with As metabolism were investigated in P. vittata after growing 60-day with Rhizophagus irregularis inoculation under 0 (As0), 10 (As10), or 100 μM As (As100) treatments. Based on the As-induced increase in AM colonization (up to 21%), AM symbiosis promoted P. vittata growth by 24% and frond P content by 22% in the AM+As100 treatment than As100 treatment. These increases corresponded to 4.2- to 5.4-fold upregulation in symbiotic P transporter RiPT1/7 in AM fungi and PvPht1;6 in P. vittata roots, which probably supported 37% greater As accumulation at 4980 mg kg–1 in the fronds. Besides total As, enhanced arsenate reduction was evidenced by 19% greater arsenite and 15-fold upregulation of fungal arsenate reductase RiArsC in mycorrhizal roots. Further, the 2.1-fold upregulation of arsenite antiporters PvACR3/3;3 contributed to greater arsenite translocation to and sequestration in the fronds. Unlike P. ensiformis symbiont, which suffers from As stress, the mycorrhiza-specific P transporters (RiPT1/7 and PvPht1;6), arsenate reductases (RiArsC and PvHAC2), and arsenite antiporters (PvACR3/3;3) all benefited AM symbiosis and As accumulation in P. vittata.