Photothermal visual sensing of alkaline phosphatase based on the etching of Au@MnO2 core-shell nanoparticles

光热治疗 抗坏血酸 检出限 纳米颗粒 化学 碱性磷酸酶 纳米技术 蚀刻(微加工) 温度计 材料科学 核化学 组合化学 色谱法 生物化学 量子力学 物理 食品科学 图层(电子)
作者
Jin Li,Xiaopeng Liu,Wenqi Ye,Zhang‐Run Xu
出处
期刊:Journal of Colloid and Interface Science [Elsevier BV]
卷期号:641: 568-576 被引量:6
标识
DOI:10.1016/j.jcis.2023.03.091
摘要

Alkaline phosphatase (ALP), as a crucial enzyme involved in many physiological activities, is always used as one of the significant biomarkers in clinical diagnosis. Herein, a novel, simple, and effective photothermal quantitative method based on the etching of MnO2-coated gold nanoparticles (Au@MnO2 NPs) was established for ALP activity assay with a household thermometer-based visual readout. The photothermal effect of Au@MnO2 NPs is much higher than that of MnO2 NPs or Au NPs. The MnO2 shell of Au@MnO2 NPs can be etched by ascorbic acid, a product of ALP-catalyzed hydrolysis of 2-phospho-l-ascorbic acid. With the etching of Au@MnO2 NPs, the photothermal conversion efficiency decreased gradually, causing the decrease of the temperature increment of the solutions by degrees. A household thermometer, instead of large-scale and professional instruments, was used as a signal reader to realize the visual quantitative detection. The photothermal platform was used successfully for the determination of ALP with a wide linear range from 2.0 to 50 U/L and a detection limit as low as 0.75 U/L. Moreover, the inhibition efficiency of sodium vanadate for ALP activity was investigated, proving the photothermal quantitative method will be a potential platform for screening enzyme inhibitors. Such a sensitive, facile, and low-cost sensing assay provides a new prospect to develop platforms for point-of-care testing.
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