固态发酵
纤维二糖
发酵
化学
食品科学
生物量(生态学)
酶
水解
生物化学
玉米芯
纤维素酶
生物
有机化学
农学
原材料
作者
Meena Sankar,Reshma M. Mathew,Anoop Puthiyamadam,AthiraRaj Sreeja-Raju,Meera Christopher,D. V. Gokhale,Rajeev Sukumaran
标识
DOI:10.1016/j.biortech.2023.128602
摘要
Solid-state fermentation (SSF) and submerged fermentation (SmF) have often been compared for production of biomass hydrolyzing enzymes highlighting the superiority of the SSF produced enzymes, but the reasons for the performance differences are under-explored. Penicillium janthinellum NCIM 1366 culture extracts from SSF had better hydrolytic performance along with a higher initial rate of reaction. Secretome analyses of the SSF and SmF enzymes using LC/MS-MS, indicated that while the type of proteins secreted were similar in both modes, the abundance of specific beta glucosidases, lytic polysaccharide monooxygenases and hemicellulolytic enzymes were very high in SSF resulting in efficient initiation, low accumulation of cellobiose and high initial reaction rates. Key enzymes that catalyse lignocellulose breakdown under SSF and SmF are therefore different and the fungus may be speculated to have regulation mechanisms that aid differential expression under different cultivation modes.
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