Nanoplastics affect the growth of sea urchins (Strongylocentrotus intermedius) and damage gut health

Nanoplastics (NPs) are abundant and widespread throughout the ocean, not only causing severe environmental pollution, but also worsening the aquatic organisms. To elucidate the mechanism of biological toxic effects underlying the responses of marine invertebrates to NPs, Strongylocentrotus intermedius was stressed with three different NPs concentrations (0 particles/L, 102 particles/L and 104 particles/L). Specific growth rates, enzyme activity, gut tissue section observation and structural characteristics of the gut bacterial community were analyzed. After 28 days of exposure, the specific growth rate of S. intermedius decreased significantly with NPs groups. Further, both lysozyme, pepsin, lipase and amylase activities decreased, while the superoxide dismutase activity increased, indicating that NPs negatively affected digestive enzyme and immune enzyme activity. The analysis of gut tissue sections revealed that NPs caused atrophy and cytoplasmic reduction in the epithelial cells of the S. intermedius intestine. Moreover, the structural characterization of the gut bacterial community indicated significant changes in the abundances of members from Campylobacterota, Chlamydiae, and Firmicutes. Members from Arcobacteraceae, Christensenellaceae and Clostridia were endemic to the NPs treatment. The KEGG database analysis demonstrated that the metabolic pathways specific to the NPs treatment group were significantly associated with growth, energy metabolism, and immunity. In summary, NPs have negatively affected on physiological response and altered gut microecological environment.