Honey and Aloe vera Solution Increases Survival and Modulates the Tumor Size In Vivo

Scope The combination of honey and Aloe vera is used as a popular complementary treatment for cancer due to their nutraceutical properties. This study aims to investigate the anticancer activity of honey and A. vera solution and its ethanolic extraction through in vitro and in vivo approaches. Methods and results After comparisons of honey and A. vera (HA) solution and its ethanolic extraction solution (E) samples by UPLC‐ESI‐MS/MS, the study verifies HA‐treatment affected only Walker tumor cells viability at the highest dose, and E‐treatment has a more cytotoxic/antiproliferative effect in MCF‐7 and Walker‐256 cells. The in vivo results show a higher survival rate in Walker‐256 tumor‐bearing rats (WHA), with higher NK cell infiltration in tumor tissue and a tendency in the WE group. These results are possible due to decreased mannose‐based immunomodulatory polysaccharides and aloin‐A contents in the ethanolic extract solution compared to HA solution. Conclusion The current study provides compelling evidence of selectively cytotoxic against tumor cells under honey and A. vera solution and ethanolic extraction solution treatment, due to the cytotoxic/antiproliferative compounds. Therefore, the use of honey and A. vera solution could be used as a basis for coadjuvant therapy in cancer treatment.