蔗糖
果糖
转化酶
活性氧
生物
龙葵
雄蕊
花粉
程序性细胞死亡
脱落酸
胞浆
细胞生物学
糖
不育
植物
生物化学
基因
细胞凋亡
酶
作者
Qiongqiong Zhang,Xi Wang,Tianying Zhao,Junfeng Luo,Xin Liu,Jing Jiang
标识
DOI:10.1093/plphys/kiae374
摘要
Abstract Cytosolic invertase (CIN) in plants hydrolyzes sucrose into fructose and glucose, influencing flowering time and organ development. However, the underlying molecular mechanisms remain elusive. Through expressional, genetic, and histological analyses, we identified a substantially role of SlCIN2 (localized in mitochondria) in regulating flowering and pollen development in tomato (Solanum lycopersicum). The overexpression of SlCIN2 resulted in increased hexose accumulation and decreased sucrose and starch content. Our findings indicated that SlCIN2 interacts with Sucrose transporter2 (SlSUT2) to inhibit the sucrose transport activity of SlSUT2, thereby suppressing sucrose content in flower buds and delaying flowering. We found that higher levels of glucose in SlCIN2-overexpressing anthers result in the accumulation of abscisic acid (ABA) and reactive oxygen species (ROS), thereby disrupting programmed cell death (PCD) in anthers and delaying the end of tapetal degradation. Exogenous sucrose partially restored fertility in SlCIN2-overexpressing plants. This study revealed the mechanism by which SlCIN2 regulates pollen development and demonstrated a strategy for creating sugar-regulated gene male sterility lines for tomato hybrid seed production.
科研通智能强力驱动
Strongly Powered by AbleSci AI