亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Gegen Qinlian standard decoction alleviated irinotecan-induced diarrhea via PI3K/AKT/NF-κB axis by network pharmacology prediction and experimental validation combination

伊立替康 汤剂 医学 药理学 PI3K/AKT/mTOR通路 蛋白激酶B 针灸科 传统医学 内科学 化学 信号转导 结直肠癌 替代医学 癌症 病理 生物化学
作者
Jiamei Chen,Min Li,Rong Chen,Ziyi Xu,Xiaoqin Yang,Huan Gu,Lele Zhang,Chaomei Fu,Jinming Zhang,Yihan Wu
出处
期刊:Chinese Medicine [BioMed Central]
卷期号:18 (1) 被引量:8
标识
DOI:10.1186/s13020-023-00747-3
摘要

Abstract Background The frequently occurred chemotherapy-induced diarrhea (CID) caused by irinotecan (CPT-11) administration has been the most representative side-effects of CPT-11, resulting in the chemotherapy suspension or failure. Our previous studies indicated that Gegen Qinlian formula exhibited a significant alleviation effect on CPT-11-induced diarrhea. However, referencing to Japanese Kampo medicine, the TCM standard decoction would supply the gap between ancient preparation application and modern industrial production. Methods The LC–MS technology combined with network pharmacology was employed to identify the active ingredients and mechanisms of GQD standard decoction for CPT-11-induced diarrhea. The anti-inflammatory activities associated with intestinal barrier function of GQD standard decoction were studied by SN-38 activated NCM460 cells in vitro and CPT-11-induced diarrhea in vivo. Proteins involved in inflammation, mRNA levels, disease severity scores, and histology involved in intestinal inflammation were analysed. Results There were 37 active compounds were identified in GQD standard decoction. Network pharmacology analyses indicated that PI3K-AKT signaling pathway were probably the main pathway of GQD standard decoction in CPT-11-induced diarrhea treatment, and PIK3R1, AKT1, NF-κB1 were the core proteins. Moreover, we found that the key proteins and pathway predicted above was verified in vivo and in vitro experiments, and the GQD standard decoction could protect the cellular proliferation in vitro and ameliorate CPT-11-induced diarrhea in mice model. Conclusions This study demonstrated the molecular mechanism of 37 active ingredients in GQD standard decoction against CPT-11-induced diarrhea. And the core proteins and pathway were validated by experiment. This data establishes the groundwork for particular molecular mechanism of GQD standard decoction active components, and this research can provide a scientific reference for the TCM therapy of CID.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
万能图书馆应助zzy采纳,获得10
12秒前
39秒前
42秒前
45秒前
Good39发布了新的文献求助10
45秒前
科研通AI2S应助科研通管家采纳,获得10
49秒前
50秒前
1分钟前
Omni发布了新的文献求助20
1分钟前
1分钟前
1分钟前
ausue发布了新的文献求助30
1分钟前
1分钟前
1分钟前
小蘑菇应助布鲁爱思采纳,获得10
1分钟前
1分钟前
aiiLnT发布了新的文献求助10
1分钟前
1分钟前
huhu完成签到,获得积分10
2分钟前
2分钟前
2分钟前
2分钟前
longsiping发布了新的文献求助10
2分钟前
2分钟前
2分钟前
胡萝卜叶子完成签到,获得积分10
2分钟前
科研通AI5应助longsiping采纳,获得10
2分钟前
Hello应助天真咖啡豆采纳,获得10
2分钟前
Li应助科研通管家采纳,获得10
2分钟前
bc应助科研通管家采纳,获得20
2分钟前
无花果应助科研通管家采纳,获得10
2分钟前
zho发布了新的文献求助10
2分钟前
3分钟前
3分钟前
布鲁爱思发布了新的文献求助10
3分钟前
星辰大海应助布鲁爱思采纳,获得10
3分钟前
zzy发布了新的文献求助10
3分钟前
大胆的渊思完成签到 ,获得积分10
3分钟前
3分钟前
3分钟前
高分求助中
Introduction to Strong Mixing Conditions Volumes 1-3 500
Tip60 complex regulates eggshell formation and oviposition in the white-backed planthopper, providing effective targets for pest control 400
Optical and electric properties of monocrystalline synthetic diamond irradiated by neutrons 320
共融服務學習指南 300
Essentials of Pharmacoeconomics: Health Economics and Outcomes Research 3rd Edition. by Karen Rascati 300
Peking Blues // Liao San 300
Political Ideologies Their Origins and Impact 13 edition 240
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 物理 生物化学 纳米技术 计算机科学 化学工程 内科学 复合材料 物理化学 电极 遗传学 量子力学 基因 冶金 催化作用
热门帖子
关注 科研通微信公众号,转发送积分 3800920
求助须知:如何正确求助?哪些是违规求助? 3346432
关于积分的说明 10329313
捐赠科研通 3062993
什么是DOI,文献DOI怎么找? 1681298
邀请新用户注册赠送积分活动 807463
科研通“疑难数据库(出版商)”最低求助积分说明 763714