漆酶
阿布茨
化学
激进的
磺胺嘧啶
磺胺二甲氧嘧啶
核化学
色谱法
有机化学
抗氧化剂
生物化学
抗生素
酶
DPPH
作者
Binbin Ouyang,Wei Xu,Wenli Zhang,Cuie Guang,Wanmeng Mu
标识
DOI:10.1016/j.jece.2022.108809
摘要
The laccase-mediator system (LMS) is an important solution to eliminate veterinary residues. In this study, a novel laccase from Lysinibacillus fusiformis (Lyfu-Lac) showed promising removal efficiency towards sulfonamides and tetracyclines residues in the presence of syringic acid (SA) and 2'-Azinobis-3-ethylbenzthiazoline-6-sulphonate (ABTS). The optimum temperature of Lyfu-Lac against ABTS was 80 °C, which is much higher than that of most laccases. At 40 °C, more than 85% of sulfamethazine (SMZ), sulfamethoxazole (SMX), and sulfadiazine (SDZ) were removed by the Lyfu-Lac-SA system after 12 h. Meanwhile, tetracycline (TC), and oxytetracycline (OTC) were significantly reduced to 0% and 16.9% by the Lyfu-Lac-ABTS system after 6 h. Based on the analysis of transformation products (TPs), a two-step reaction was reasonably proposed in the removal of sulfonamides: (i) the oxidation of mediators by Lyfu-Lac to radicals, (ii) the adductive reaction between radicals and sulfonamides. Moreover, the removal mechanism of tetracyclines was proposed, including the cleavage of the N-N bond after the adductive reaction between ABTS radicals and tetracyclines.
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