Multi-component pharmacokinetic study of prunus mume fructus extract after oral administration in rats using UPLC-MS/MS

化学 色谱法 绿原酸 咖啡酸 原儿茶酸 丁香酸 甲酸 蛋白质沉淀 香兰素酸 阿魏酸 高效液相色谱法 奎宁酸 甘草苷 芦丁 酚酸 串联质谱法 柠檬酸 没食子酸 质谱法 生物化学 抗氧化剂
作者
Yameng Zhu,Shujie Wei,Xiunan Cao,Songrui Wang,Yanxu Chang,Huizi Ouyang,Jun He
出处
期刊:Frontiers in Pharmacology [Frontiers Media SA]
卷期号:13
标识
DOI:10.3389/fphar.2022.954692
摘要

Prunus mume fructus (MF) is used in traditional Chinese medicine and food, as it exerts pharmacological effects, such as antibacterial, antioxidant, antitumour, thirst-relieving, and antidiarrheal effects. In the present study, a reliable and sensitive ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the simultaneous determination of 16 prototype components (L-(-)-malic acid, 3,4-dihydroxybenzaldehyde, protocatechuic acid, vanillic acid, caffeic acid, D-(-)-quinic acid, citric acid, ferulic acid, syringic acid, cryptochlorogenic acid, neochlorogenic acid, chlorogenic acid, amygdalin, maslinic acid, corosolic acid, and rutin) in rat plasma after oral administration of the MF extract. Plasma samples were prepared via protein precipitation using acetonitrile. The 16 components were separated on an ACQUITY UPLC BEH C18 column (2.1 × 100 mm, 1.7 μm) with a gradient mobile phase system of methanol and 0.1% (v/v) formic acid aqueous solution at a flow rate of 0.3 ml/min. All components were quantitated using Agilent Jet Stream electrospray ionisation in negative ion mode. The intra-day and inter-day accuracies ranged from-9.4 to 9.4%, and the precision of the analytes was less than 14.8%. The extraction recovery rate of the analytes ranged from 63.59 to 109.44% and the matrix effects ranged from 49.25 to 109.28%. Stability studies proved that the analytes were stable under the tested conditions, with a relative standard deviation lower than 13.7%. Hence, the developed method was successfully applied to evaluate the pharmacokinetics of 16 components in the MF extract after oral administration in rats using UPLC-MS/MS.
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