脂磷壁酸
溶解循环
溶解
酶
肽聚糖
细菌细胞结构
抗菌剂
抗菌肽
流出
膜
生物化学
微生物学
化学
细胞膜
行动方式
生物
金黄色葡萄球菌
细菌
免疫学
病毒
遗传学
作者
Miriam Wilmes,Marina Stockem,Gabriele Bierbaum,Martin Schlag,Friedrich Götz,Dat Q. Tran,Justin B. Schaal,André J. Ouellette,Michael E. Selsted,Hans-Georg Sahl
出处
期刊:Antibiotics
[MDPI AG]
日期:2014-11-14
卷期号:3 (4): 617-631
被引量:36
标识
DOI:10.3390/antibiotics3040617
摘要
θ-Defensins are cyclic antimicrobial peptides expressed in leukocytes of Old world monkeys. To get insight into their antibacterial mode of action, we studied the activity of RTDs (rhesus macaque θ-defensins) against staphylococci. We found that in contrast to other defensins, RTDs do not interfere with peptidoglycan biosynthesis, but rather induce bacterial lysis in staphylococci by interaction with the bacterial membrane and/or release of cell wall lytic enzymes. Potassium efflux experiments and membrane potential measurements revealed that the membrane impairment by RTDs strongly depends on the energization of the membrane. In addition, RTD treatment caused the release of Atl-derived cell wall lytic enzymes probably by interaction with membrane-bound lipoteichoic acid. Thus, the premature and uncontrolled activity of these enzymes contributes strongly to the overall killing by θ-defensins. Interestingly, a similar mode of action has been described for Pep5, an antimicrobial peptide of bacterial origin.
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