Isoflavones with neuroprotective activities from fruits of Cudrania tricuspidata

Ten isoflavones, cudraisoflavones B–K, together with 27 known isoflavones, were isolated from the EtOAc soluble extract of the fruits of Cudrania tricuspidata . Six of these (compounds 7 – 9 and 11 – 13 ) showed neuroprotective activity against 6-hydroxydopamine induced cell death in human neuroblastoma SH-SY5Y cells, with EC 50 values of 0.5–9.2 μM. • 37 isoflavones, 27 previously known, were isolated from Cudrania tricuspidata . • Their structures were elucidated by HRESIMS, 1D and 2D NMR spectroscopic data. • Six of the isoflavones showed therapeutic potential in treating neurotoxicity. Ten isoflavones, cudraisoflavones B–K ( 1 – 10 ), together with 27 known isoflavones, were isolated from the EtOAc soluble extract of fruits of Cudrania tricuspidata . The structures of compounds 1 – 10 were elucidated on the basis of MS and NMR spectroscopic data, including 2D NMR experiments. Compounds 7 – 9 and three known ( 11 – 13 ) compounds showed neuroprotective activity against 6-hydroxydopamine induced cell death in human neuroblastoma SH-SY5Y cells, with EC 50 values of 0.5–9.2 μM.