淋巴管新生
平足蛋白
淋巴
医学
病理
淋巴系统
淋巴管
淋巴结
血管生成
淋巴管内皮
转移
血管内皮生长因子C
内皮糖蛋白
免疫组织化学
癌症研究
癌症
血管内皮生长因子
生物
内科学
血管内皮生长因子A
川地34
干细胞
血管内皮生长因子受体
遗传学
作者
Sílvia Ferreira de Sousa,Frederico O. Gleber‐Netto,Helenisa Helena Oliveira-Neto,Aline Carvalho Batista,Mauro Henrique Nogueira Guimarães de Abreu,Maria Cássia Ferreira de Aguiar
出处
期刊:Applied Immunohistochemistry & Molecular Morphology
日期:2012-12-01
卷期号:20 (6): 588-594
被引量:29
标识
DOI:10.1097/pai.0b013e31824bb3ea
摘要
The objective of this study was to evaluate lymphangiogenesis in oral squamous cell carcinoma and in the associated lymph nodes and podoplanin expression in neoplastic cells at the invasive front. In addition, the association of the above parameters with lymph node metastasis was also investigated. We used immunohistochemistry to examine primary tumors and lymph nodes, regardless of metastasis. Lymphatic vessel density (LVD) and microvessel density (MVD) were assessed by antibodies D2-40 and CD105, respectively, in intratumoral and peritumoral areas and in lymph node regions. Vascular endothelial growth factor-C and vascular endothelial growth factor receptor-3 expression was evaluated in tumor cells and D2-40 (podoplanin) expression in parenchymal cells found at the invasive front. The majority of cases with nodal involvement presented a high peritumoral LVD. In addition, a strong association of LVD with size and site of primary tumors could also be identified. MVD was statistically associated with metastasis, and a significant association between the lymphangiogenic factors and the density of vessels in the intratumoral region was also seen. The well-differentiated tumors did not express podoplanin. LVD and MVD were higher in metastatic lymph nodes than in nonmetastatic lymph nodes. The enhanced vascular network in metastatic lymph nodes reinforces the previous reports of lymphangiogenesis occurrence in lymph nodes. Moreover, the expression of podoplanin by more undifferentiated tumor cells suggests that this protein could be an indicator of tumor aggressiveness.
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