小角X射线散射
管道(软件)
吞吐量
火球菌属
结构基因组学
散射
材料科学
结晶学
计算生物学
生物系统
计算机科学
化学
蛋白质结构
物理
生物
光学
生物化学
基因
古细菌
电信
程序设计语言
无线
作者
Greg L. Hura,Angeli Lal Menon,Michal Hammel,Robert P. Rambo,Farris L. Poole,Susan E. Tsutakawa,Francis E. Jenney,Scott Classen,Kenneth A. Frankel,Robin Hopkins,Sung Jae Yang,Joseph W. Scott,B.D. Dillard,Michael W. W. Adams,John A. Tainer
出处
期刊:Nature Methods
[Springer Nature]
日期:2009-07-20
卷期号:6 (8): 606-612
被引量:625
摘要
We present an efficient pipeline enabling high-throughput analysis of protein structure in solution with small angle X-ray scattering (SAXS). Our SAXS pipeline combines automated sample handling of microliter volumes, temperature and anaerobic control, rapid data collection and data analysis, and couples structural analysis with automated archiving. We subjected 50 representative proteins, mostly from Pyrococcus furiosus, to this pipeline and found that 30 were multimeric structures in solution. SAXS analysis allowed us to distinguish aggregated and unfolded proteins, define global structural parameters and oligomeric states for most samples, identify shapes and similar structures for 25 unknown structures, and determine envelopes for 41 proteins. We believe that high-throughput SAXS is an enabling technology that may change the way that structural genomics research is done.
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