Temperature-responsive hydroxybutyl chitosan for the culture of mesenchymal stem cells and intervertebral disk cells

材料科学 生物材料 聚合物 壳聚糖 间充质干细胞 粘弹性 化学工程 组织工程 生物物理学 生物医学工程 纳米技术 复合材料 医学 生物 细胞生物学 工程类
作者
Jiyoung M. Dang,Daniel Sun,Yoshitsune Shin‐ya,Ann N. Sieber,John P. Kostuik,Kam W. Leong
出处
期刊:Biomaterials [Elsevier BV]
卷期号:27 (3): 406-418 被引量:228
标识
DOI:10.1016/j.biomaterials.2005.07.033
摘要

Temperature-responsive polymers are attractive candidates for applications related to injectable delivery of biologically active therapeutics, such as stem cells. In this study, we evaluate the potential of thermosensitive hydroxybutyl chitosan (HBC) as a biomaterial for the culture of human mesenchymal stem cells (hMSC) and cells derived from the intervertebral disk, with the eventual goal of using the HBC polymer as an injectable matrix/cell therapeutic. Conjugation of hydroxybutyl groups to chitosan renders the polymer water soluble and thermally responsive. Below its lower critical solution temperature, a solution of HBC can be maintained indefinitely in its solvated state. Upon exposure to a 37 degrees C environment, within 60 s, a 3.8 wt% HBC solution rapidly forms a gel that can be maneuvered with forceps. Upon cooling, the gel once again is able to revert to its solvated state. The gel exhibits a dramatic increase in both G' and G'' with increasing temperature, signifying a temperature-dependent enhancement of gel mechanical properties. Although a solid structure upon gelation, due to its physical nature of polymer interaction and gel formation, the gel exhibits a fluid-like viscoelastic behavior when exposed to shear stresses of up to 10% strain, with both G' and G'' approaching zero with increasing shear stress. Formulations of HBC gels presented in this study have gelation temperatures ranging from 13.0 to 34.6 degrees C and water contents of 67-95%. Minimal cytotoxicity in MSC and disk cell cultures was observed with these polymers up to a concentration of 5 wt%. Detection of metabolic activity, genetic analysis of synthesized mRNA, and histological staining of MSC and disk cell cultures in these gels collectively indicate cell proliferation without a loss in metabolic activity and extracellular matrix production. This study suggests the potential of HBC gel as an injectable carrier for future applications of delivering therapeutics to encourage a biologically relevant reconstruction of the degenerated disk.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
李xue发布了新的文献求助10
1秒前
1秒前
宇老师完成签到,获得积分10
2秒前
复杂之桃关注了科研通微信公众号
3秒前
3秒前
4秒前
过时的哑铃完成签到,获得积分10
4秒前
甜美代秋发布了新的文献求助10
5秒前
为你博弈发布了新的文献求助10
5秒前
御剑乘风来完成签到,获得积分10
5秒前
魏冉发布了新的文献求助10
5秒前
YK完成签到,获得积分10
7秒前
7秒前
Spike发布了新的文献求助20
7秒前
largedream发布了新的文献求助30
7秒前
xwzz发布了新的文献求助10
8秒前
8秒前
8秒前
8秒前
张三完成签到,获得积分10
8秒前
8秒前
超级青荷完成签到,获得积分10
8秒前
9秒前
Zzz完成签到,获得积分10
9秒前
诗图完成签到,获得积分10
9秒前
风中的逍遥完成签到,获得积分10
9秒前
you发布了新的文献求助10
10秒前
fairy发布了新的文献求助20
10秒前
10秒前
kento发布了新的文献求助30
10秒前
治神守气发布了新的文献求助10
11秒前
铁铁发布了新的文献求助10
12秒前
12秒前
13秒前
14秒前
执着从灵完成签到 ,获得积分10
14秒前
BK_关注了科研通微信公众号
14秒前
14秒前
15秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Picture Books with Same-sex Parented Families: Unintentional Censorship 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 360
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3970802
求助须知:如何正确求助?哪些是违规求助? 3515474
关于积分的说明 11178714
捐赠科研通 3250627
什么是DOI,文献DOI怎么找? 1795390
邀请新用户注册赠送积分活动 875818
科研通“疑难数据库(出版商)”最低求助积分说明 805183