谷氨酰胺合成酶
生物
氮气循环
氮气
苗木
抗氧化剂
铵
褪黑素
新陈代谢
氮同化
脂质过氧化
酶
生物化学
植物
谷氨酰胺
化学
内分泌学
氨基酸
有机化学
作者
Huamei Wang,Chunyuan Ren,Liang Cao,Xijun Jin,Mengxue Wang,Mingcong Zhang,Qiang Zhao,He Li,Yuxian Zhang,Gaobo Yu
摘要
To investigate the function of melatonin (MT) on nitrogen uptake and metabolism in soybean, six groups of treatments, with and without 100 μM melatonin were conducted at low, normal, and high nitrogen levels (1.5, 7.5, and 15 mM, respectively). The related indexes of nitrogen metabolism and the antioxidant system of seedlings were measured and analysed. Results indicated that MT could enhance the level of nitrogen metabolism by upregulating the coding genes of enzymes related to nitrogen metabolism and increasing total nitrogen content, especially under low nitrogen levels. Under high nitrogen conditions, the addition of MT not only accelerated ammonium assimilation and utilisation by enhancing the activity of glutamine synthetase involved in ammonium assimilation, but also reduced the extent of membrane lipid peroxidation to alleviate the degree of damage by improving the activity of antioxidant enzymes. In addition, MT enhanced soybean growth with positive effects in morphological changes at different nitrogen levels, including significantly increased stem diameter, total leaf area, and root nodule number, and biomass accumulation. Finally, biomass accumulation increased under low, normal, and high nitrogen levels by 9.80%, 14.06%, and 11.44%, respectively. The results suggested that MT could enhance the soybean tolerance to low and excessive N treatments.
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