Microalgae cultivation using unsterilized cattle farm wastewater filtered through corn stover

The aim of this study was to investigate the cultivation of Chlorella sp. (FACHB-8) and Kirchneriella obesa (FACHB-2104) using the unsterilized cattle farm wastewater (CFW) filtered through corn stover. Corn stover filtration effectively reduced the turbidity and suspended solids of CFW and improved the adaptability of microalgae to CFW. The yields of microalgae supplemented with filtered CFW were significantly higher than those of microalgae supplemented with unfiltered CFW-by 14%-57% (FACHB-8) and 12%-78% (FACHB-2104) and comparable to those with pure blue-green algae medium (BG11). The growth kinetics of microalgae conformed to the DoseResp model. A 3:6 ratio of filtered CFW to BG11 and an 8000 lx light intensity were optimal for achieving high microalgae production. Under optimum conditions, the maximal yields of FACHB-8 and FACHB-2104 were 1.26 and 1.22 g/L, respectively, and the removal efficiencies of nitrogen, phosphorus, and chemical oxygen demand exceeded 95%, 99%, and 82%, respectively.