Review of Clinical Performance of Serology Based Commercial Diagnostic Assays for Detection of Severe Acute Respiratory Syndrome Coronavirus 2 Antibodies

血清学 医学 金标准(测试) 抗体 病毒学 免疫分析 人口 免疫学 大流行 冠状病毒 疾病 2019年冠状病毒病(COVID-19) 传染病(医学专业) 内科学 环境卫生
作者
Poonam S. Deshpande,Irene E. Abraham,Anjali Pitamberwale,R Dhôte
出处
期刊:Viral Immunology [Mary Ann Liebert]
卷期号:35 (2): 82-111 被引量:4
标识
DOI:10.1089/vim.2020.0313
摘要

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, which caused the coronavirus disease 2019 (COVID-19) pandemic as declared by the World Health Organization, has created havoc worldwide. The highly transmissible infection can be contained only by accurate diagnosis, quarantining, and exercising social distancing. Therefore, quick and massive deployment of SARS-CoV-2 testing plays a crucial role in the identification and isolation of infected patients. Reverse transcription-polymerase chain reaction is the gold standard for COVID-19 detection; however, it needs expertise, facilities, and time. Hence, for the ease of population-wide screening, serology-based diagnostic assays were introduced. These can help determine the prevalence of infection, understand the epidemiology of the disease, and assist in suitable public health interventions while being user-friendly and less time consuming. Although serological testing kits in markets soared, their sensitivity and specificity were questioned in reports from different parts of the world. In this article, we have reviewed 40 Food and Drug Administration (FDA) and CE-approved clinically evaluated serological kits (8 enzyme-linked immunosorbent assay [ELISA] kits, 10 chemiluminescent immunoassay [CLIA] kits, and 22 lateral flow immunoassay [LFIA] kits) for their sensitivity and specificity and discussed the apparent reasons behind their performance. We observed appreciable sensitivity in the kits detecting total antibodies compared to the kits targeting single isotype antibodies. Tests that determined antibodies against nucleocapsid protein were found to be more sensitive and those detecting antibodies against spike protein were found to have greater specificity. This study was conducted to help the decision-making while acquiring antibody kits and concurrently to be mindful of their shortcomings.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
lala完成签到,获得积分10
刚刚
6188完成签到 ,获得积分10
刚刚
比奇堡艺术家完成签到,获得积分10
刚刚
LYL完成签到,获得积分10
刚刚
Yjj发布了新的文献求助10
1秒前
开门啊菇凉完成签到,获得积分0
1秒前
百里盼山完成签到,获得积分20
1秒前
nn完成签到 ,获得积分10
1秒前
2秒前
2秒前
出门见喜完成签到,获得积分10
3秒前
爱听歌火龙果完成签到,获得积分20
3秒前
wmzskye完成签到,获得积分10
3秒前
4秒前
强强强强完成签到,获得积分10
5秒前
欣喜的莫茗完成签到 ,获得积分10
6秒前
一路向阳完成签到,获得积分10
6秒前
不知道发布了新的文献求助10
6秒前
Aki_27完成签到,获得积分10
7秒前
爱科研完成签到,获得积分10
7秒前
风夏完成签到,获得积分10
7秒前
owoow发布了新的文献求助10
8秒前
炙热初晴发布了新的文献求助10
9秒前
落幕熊猫完成签到,获得积分10
9秒前
于芋菊完成签到,获得积分0
9秒前
9秒前
宜醉宜游宜睡完成签到,获得积分0
9秒前
科研通AI2S应助百里盼山采纳,获得10
11秒前
buno应助敏感的鼠标采纳,获得10
11秒前
rainny完成签到,获得积分10
11秒前
结实的凉面完成签到,获得积分10
12秒前
于芋菊发布了新的文献求助10
12秒前
Owen应助Bag633采纳,获得10
12秒前
细腻依云完成签到,获得积分10
12秒前
丘比特应助mm采纳,获得10
14秒前
Annabelame完成签到,获得积分10
14秒前
老乡开下门吧完成签到,获得积分10
16秒前
zsy完成签到,获得积分10
17秒前
科研通AI2S应助研友_8KX15L采纳,获得10
17秒前
传奇3应助绿洲采纳,获得10
18秒前
高分求助中
The late Devonian Standard Conodont Zonation 2000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Smart but Scattered: The Revolutionary Executive Skills Approach to Helping Kids Reach Their Potential (第二版) 1000
Very-high-order BVD Schemes Using β-variable THINC Method 850
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 800
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3249053
求助须知:如何正确求助?哪些是违规求助? 2892387
关于积分的说明 8271490
捐赠科研通 2560723
什么是DOI,文献DOI怎么找? 1389228
科研通“疑难数据库(出版商)”最低求助积分说明 651029
邀请新用户注册赠送积分活动 627870