植物甾醇
生物催化
脂肪酶
化学
南极洲假丝酵母
水解
皮克林乳液
介孔材料
化学工程
有机化学
介孔二氧化硅
酶
固定化酶
乳状液
催化作用
离子液体
色谱法
工程类
作者
Tao Yang,Yufei Zhang,Liujia Xu,Hao Zhu,Juntao Zhang,Zheng Guo,Fenghong Huang,Mingming Zheng
出处
期刊:ACS Sustainable Chemistry & Engineering
[American Chemical Society]
日期:2022-05-17
卷期号:10 (21): 6963-6972
被引量:18
标识
DOI:10.1021/acssuschemeng.1c08719
摘要
Herein, a pH-switchable Pickering interfacial biocatalysis (PIB) system is proposed and applied to a solvent-free biphasic biocatalysis. The oil-in-water emulsion was stabilized by amine-functionalized hollow mesoporous silica nanospheres (HMSS-N), which is functioned as emulsifiers of PIB and carriers of lipase AYS. The smart emulsifiers exhibited switch of the surface wettability behaviors in response to abrupt changes of pH. Under the optimality conditions, the immobilized lipase loading amount, activity, and expressed activity were 174.1 mg g–1, 9073 U g–1, and 52.1 U g–1 protein, respectively, at pH 6. Based on it, the hydrolysis of rice bran oil and esterification with phytosterols to produce phytosterol esters (PEs) were successfully performed. Compared with the conventional free enzyme one-phase system, the PIB system displays a higher reaction efficiency (catalytic efficiency value 6.88 mmol g–1 h–1, 20-fold enhancement). In addition, the system maintains 95% residual activity after 10 reaction cycles with 38.3 g products/g biocatalyst average productivity, complying with the requirements of industry-accepted standards. In each cycle, the demulsification is realized simply by in situ pH regulation. Therefore, this paper provides a powerful and green platform for oil–fat modification and stimuli-responsive interfacial biocatalysis with the advantages of a high efficiency, sustainability, a solvent-free process, and multi-recyclability.
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