Fluorescence Turn‐On Detection of Nitric Oxide in Aqueous Solution Using Cationic Conjugated Polyelectrolytes

Abstract A fluorescent turn‐on detection for nitric oxide in aqueous solution is developed using cationic conjugated polymers. The assay benefits from the sensitivity of optical signals from conjugated polymers and the simplicity of fluorescence measurement techniques. The assay contains three elements: a cationic conjugated polymer that contains imidazole moieties, Cu 2+ ions, and the target nitric oxide. The highly fluorescent conjugated polymer coordinates to Cu 2+ ions through weak N · · · Cu interactions, and its fluorescence is efficiently quenched by a photo‐induced electron transfer process (‘off’ state). In the presence of nitric oxide, the transformation of the paramagnetic Cu 2+ ion into a diamagnetic Cu 1+ ion inhibits the quenching and, therefore, the fluorescence of the conjugate polymer is recovered (‘on’ state). Other biologically relevant reactive nitrogen species, such as NOBF 4 , NaNO 2 , and NaNO 3 don't exhibit the fluorescence recovery of the conjugated polymer under the same conditions as nitric oxide. The cationic conjugated polymer/Cu 2+ complex can thus be used as a platform to detect nitric oxide in aqueous solution with high sensitivity and selectivity. magnified image