Relevance between lipid metabolism-associated genes and rat liver regeneration

Lipids are important in constituting cell structure and participating in many biological processes, particularly in energy supplementation to cells. The aim of the present study is to elucidate the action of lipid metabolism-associated genes on rat liver regeneration (LR).Lipid metabolism-associated genes were obtained by collecting website data and retrieving related articles, and their expression changes in the regenerating rat liver were checked by the Rat Genome 230 2.0 array.In total, 280 genes involved in lipid metabolism were proven to be LR-associated by comparing the gene expression discrepancy between the partial-hepatectomy and sham-operation groups. The initial and total expression numbers of these genes occurring in the initial phase, G(0)/G(1) transition, cell proliferation, cell differentiation, and structure-functional rebuilding of LR were 128, 33, 135, 6, and 267, 147, 1026, 306, respectively, illustrating that these genes were initially expressed mainly in the initiation stage and functioned in different phases. Upregulation (850 times) and downregulation (749 times), as well as 25 types of expression patterns, showed that the physiological and biochemical activities were diverse and complicated in LR.According to the results of the chip detection, it was presumed that fatty acid synthesis at 24-66 h, leukotriene and androgen synthesis at 16-168 h, prostaglandin synthesis at 2-96 h, triglyceride synthesis at 18-24 h, glycosphingolipid synthesis at 0.5-66 h, metabolism of phosphatidyl inositol and sphingomyelin at 2-16 h, and cholesterol catabolism at 30-168 h were enhanced. Throughout almost the whole LR, the genes participating in estrogen, glucocorticoid, and progesterone synthesis, and triglyceride catabolism were upregulated, while phospholipid and glycosphingolipid catabolism were downregulated.