Fluorescence Resonance Energy Transfer Inhibition Assay for α-Fetoprotein Excreted during Cancer Cell Growth Using Functionalized Persistent Luminescence Nanoparticles

Persistent-luminescence nanoparticles (PLNPs) are promising as a new generation of photoluminescent probes for detection of biomolecules and bioimaging. Here we report a fluorescence resonance energy transfer (FRET) inhibition assay for α-fetoprotein (AFP) excreted during cancer cell growth using water-soluble functionalized PLNPs based on Eu2+- and Dy3+-doped Ca1.86Mg0.14ZnSi2O7. Polyethyleneimine-coated PLNPs were conjugated with AFP-antibody-coated gold nanoparticles as a sensitive and specific persistent photoluminescence probe for detection of AFP in serum samples and imaging of AFP excreted during cancer cell growth. Such PLNPs do not contain toxic heavy metals. Their long-lasting afterglow nature allows detection and imaging without external illumination, thereby eliminating the autofluorescence and scattering light from biological matrixes encountered under in situ excitation.