Detection of Respiratory Pathogens in Parapneumonic Effusions by Hypothesis-free, Next-Generation Sequencing (NGS)

医学 病菌 聚合酶链反应 一致性 微生物学 内科学 生物 基因 免疫学 遗传学
作者
Krow Ampofo,Andrew T. Pavia,Anne J. Blaschke,Robert Schlaberg
出处
期刊:Open Forum Infectious Diseases [Oxford University Press]
卷期号:4 (suppl_1): S17-S17 被引量:1
标识
DOI:10.1093/ofid/ofx162.044
摘要

Abstract Background Species-specific polymerase chain reaction (PCR) testing of pleural fluid (PF) from children with parapneumonic effusion (PPE) has increased pathogen identification in pediatric PPE. However, a pathogen is not detected in 25–35% of cases. Hypothesis-free, next-generation sequencing (NGS) provides a more comprehensive alternative and has led to pathogen detection in PCR-negative samples. However, the utility of NGS in the evaluation of PF from children with PPE is unknown. Methods Archived PF (n = 20) from children younger than 18 years with PPE and hospitalized at Primary Children’s Hospital, Utah, in 2015 and previously tested by PCR were evaluated. Ten PCR-negative and 10 PCR-positive PF specimens were tested using RNA-seq at an average depth of 7.7×106 sequencing reads per sample. NGS data were analyzed with Taxonomer. We compared pathogens detected by blood and PF culture, PCR, and NGS. Results Overall, compared with blood/PF culture, PF PCR and PF NGS testing of PF increased bacterial identification from 15% to 50% (P < 0.05) and 65% (P = 0.003), respectively. Pathogen detection in PF by PCR and NGS were comparable (50 vs. 65%, p = NS) (Table). However, compared with PF PCR, NGS significantly increased detection of S. pyogenes (20% vs. 55%; P < 0.05), with 100% concordance when detected by PCR and culture. Detection of Fusobacterium spp. (10 vs. 10%) by PF NGS and PF PCR were comparable. In contrast, there was no detection of S. pneumoniae (15 vs. 0%) by PF NGS compared with PF PCR. Conclusion PF NGS testing significantly improves bacterial identification and comparable to PF PCR testing, which can help inform antimicrobial selection. However there were differences in detection of S. pneumoniae and S. pyogenes. Further studies of NGS testing of PF of children with PPE are needed to assess its potential in the evaluation of PPE in children. Disclosures A. J. Blaschke, BioFire Diagnostics LLC: Collaborator, Have intellectual property in BioFire Diagnostics through the University of Utah and Investigator, Licensing agreement or royalty and Research support; R. Schlaberg, IDbyDNA: Co-founder, Consultant and Shareholder, Stock
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
方方完成签到 ,获得积分10
1秒前
i2stay完成签到,获得积分10
2秒前
郑雅柔完成签到 ,获得积分10
6秒前
光亮的自行车完成签到 ,获得积分10
8秒前
WXM完成签到 ,获得积分10
18秒前
《子非鱼》完成签到,获得积分10
22秒前
future完成签到 ,获得积分10
22秒前
小庄完成签到 ,获得积分10
25秒前
包子牛奶完成签到,获得积分10
33秒前
小梦完成签到,获得积分10
34秒前
不回首完成签到 ,获得积分10
41秒前
水沝完成签到 ,获得积分10
45秒前
caitSith完成签到,获得积分10
45秒前
MAKEYF完成签到 ,获得积分10
46秒前
wBw完成签到,获得积分10
53秒前
平常雨泽完成签到 ,获得积分10
54秒前
美好灵寒完成签到 ,获得积分10
57秒前
1分钟前
友好亚男完成签到 ,获得积分10
1分钟前
哈哈哈哈完成签到 ,获得积分10
1分钟前
tzy6665完成签到,获得积分10
1分钟前
Yimi刘博完成签到 ,获得积分10
1分钟前
我就想看看文献完成签到 ,获得积分10
1分钟前
唯梦完成签到 ,获得积分10
1分钟前
madison完成签到 ,获得积分10
1分钟前
lululululululu完成签到 ,获得积分10
1分钟前
vvvvvvv完成签到 ,获得积分10
1分钟前
1分钟前
1分钟前
忍冬发布了新的文献求助10
1分钟前
坚强的缘分完成签到,获得积分10
1分钟前
维生素CCC完成签到 ,获得积分10
1分钟前
执着烧鹅完成签到 ,获得积分10
1分钟前
eyu完成签到,获得积分10
1分钟前
2分钟前
听闻韬声依旧完成签到 ,获得积分10
2分钟前
zhang完成签到 ,获得积分10
2分钟前
2分钟前
微笑芒果完成签到 ,获得积分10
2分钟前
jscr完成签到,获得积分10
2分钟前
高分求助中
Evolution 10000
Sustainability in Tides Chemistry 2800
юрские динозавры восточного забайкалья 800
English Wealden Fossils 700
Diagnostic immunohistochemistry : theranostic and genomic applications 6th Edition 500
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 400
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3155031
求助须知:如何正确求助?哪些是违规求助? 2805746
关于积分的说明 7865931
捐赠科研通 2464038
什么是DOI,文献DOI怎么找? 1311698
科研通“疑难数据库(出版商)”最低求助积分说明 629734
版权声明 601862