低温保存
移植
干细胞
生物
生殖系
保持生育能力
细胞生物学
男科
免疫学
生育率
医学
胚胎
遗传学
内科学
基因
人口
环境卫生
作者
Nilam Sinha,Eoin C. Whelan,Ralph L. Brinster
出处
期刊:Methods in molecular biology
日期:2019-01-01
卷期号:: 205-220
被引量:13
标识
DOI:10.1007/978-1-4939-9524-0_14
摘要
Spermatogonial stem cell (SSC) culture and transplantation pave the way for clinical restoration of fertility in male prepubertal cancer survivors. In this chapter we detail the steps for isolating and freezing testicular tissue along with protocols for the subsequent recovery from cryopreservation and transplantation of cells into a recipient testis. Transplantation of cultured or thawed SSCs provides not only a functional assay for identification of stem cells, a critical tool for the study of the germline stem cell niche in model organisms, but also a framework for reconstitution of spermatogenesis in humans. As proof of concept, the outlined methods have been performed successfully in the murine model and have the potential to be translated to clinical environments.
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