Anode potentials regulate Geobacter biofilms: New insights from the composition and spatial structure of extracellular polymeric substances

生物膜 地杆菌 胞外聚合物 阳极 细胞外 硫化地杆菌 生物物理学 化学 胞外多糖 多糖 电子转移 电化学 化学工程 微生物燃料电池 电极 氧化还原 舍瓦内拉 微生物学 环境化学 吸附 电解质 纳米技术 细菌 材料科学 生物化学 无机化学 生物 光化学 有机化学 遗传学 物理化学 工程类
作者
Guiqin Yang,Lingyan Huang,Zhen Yu,Xiaoming Liu,Shanshan Chen,Jianxiong Zeng,Shungui Zhou,Zhuang Li
出处
期刊:Water Research [Elsevier BV]
卷期号:159: 294-301 被引量:114
标识
DOI:10.1016/j.watres.2019.05.027
摘要

The extracellular electron transfer (EET) efficiency in bioelectrochemical systems has been proven to be dependent on anode potentials. To explore the underlying mechanism, previous studies have mainly focused on EET conduit and bacterial biomass but rarely concerned with the role of extracellular polymeric substances (EPS) surrounding electroactive cells. In this study, the response of Geobacter biofilms to anode potentials was investigated with a special emphasis on the mechanistic role of EPS. The electrochemical activities and cell viabilities of Geobacter soli biofilms were simultaneously attenuated at 0.4 and 0.6 V compared to −0.2 and 0 V. It was found that the biofilms (especially the biofilm region closer to electrode surface) grown at −0.2 and 0 V produced relatively more extracellular redox-active proteins and less extracellular polysaccharides, which conferred higher electron accepting/donating capacities to EPS and consequently facilitated EET. Meanwhile, electrically nonconductive extracellular polysaccharide-dominated interior layers were formed in the biofilms grown at 0.4 and 0.6 V, which limited direct EET but might serve as physical barriers for protecting cells in these biofilms from the increasing stress by poised electrodes. These results demonstrated that the production of EPS under different anode potentials might be finely regulated by cells to keep balance between EET efficiency and cell-protection. This study provides a new insight to investigate the Geobacter biofilms coping with various environments, and is useful for optimizing electrochemical activity of anode biofilms.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Nulix完成签到,获得积分20
刚刚
刚刚
刚刚
现代茗完成签到,获得积分10
1秒前
1秒前
狡猾的菠萝完成签到 ,获得积分10
1秒前
Nulix发布了新的文献求助10
2秒前
典雅碧空应助小涛采纳,获得10
3秒前
3秒前
我是老大应助pjh采纳,获得10
4秒前
善学以致用应助用户5063899采纳,获得10
4秒前
LIHANG发布了新的文献求助10
5秒前
一起吃火锅完成签到,获得积分10
5秒前
雪白的雪完成签到,获得积分10
5秒前
5秒前
Ava应助肉肉采纳,获得10
6秒前
马上毕业完成签到 ,获得积分10
6秒前
小豆豆应助Tom采纳,获得10
7秒前
慕青应助163采纳,获得10
8秒前
8秒前
斯文白白发布了新的文献求助10
9秒前
9秒前
9秒前
wuwu关注了科研通微信公众号
9秒前
绝世镜天完成签到 ,获得积分10
11秒前
勇往直前完成签到,获得积分10
11秒前
研友_VZG7GZ应助DXXX采纳,获得10
12秒前
VV发布了新的文献求助10
12秒前
思源应助fyl采纳,获得10
13秒前
Albertxkcj发布了新的文献求助10
13秒前
Akim应助小玉采纳,获得10
13秒前
blue2021发布了新的文献求助30
14秒前
14秒前
Qing完成签到,获得积分10
15秒前
脑洞疼应助lys采纳,获得10
15秒前
yilin完成签到 ,获得积分10
15秒前
Meng完成签到,获得积分10
16秒前
思源应助o30采纳,获得10
16秒前
16秒前
ZH发布了新的文献求助10
16秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Picture Books with Same-sex Parented Families: Unintentional Censorship 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3969458
求助须知:如何正确求助?哪些是违规求助? 3514286
关于积分的说明 11173363
捐赠科研通 3249652
什么是DOI,文献DOI怎么找? 1794948
邀请新用户注册赠送积分活动 875501
科研通“疑难数据库(出版商)”最低求助积分说明 804836