A lateral flow assay for simultaneous detection of Deoxynivalenol, Fumonisin B1 and Aflatoxin B1

Deoxynivalenol (DON), Fumonisin B1 (FB1) and Aflatoxin B1 (AFB1) have strong toxicity to humans and exist widely in grain and food. It is necessary to screen these mycotoxins to guarantee food safety. In order to develop a rapid, simple, low-cost and simultaneous-detection method, composites of antibody-nano-Au-particles, DON-BSA, FB1-BSA, and AFB1-BSA were prepared to establish a lateral flow assay based on competitive inhibition. The results suggested that the visual detection limits for DON, FB1 and AFB1 were 10, 30, and 10 ng mL-1, respectively. On the other hand, it had no reactivity to T-2 toxin, zearalenone, ochratoxin A and nivalenol, which demonstrates good specificity. Besides, the strip had good repeatability and stability. Therefore, DON, FB1 and AFB1 would be screened simultaneously by lateral flow assay for food safety.