Radioprotective effect of melatonin on expression of Cdkn1a and Rad50 genes in rat peripheral blood

褪黑素 DNA修复 电离辐射 自由基清除剂 腹腔注射 DNA损伤 基因表达 雷达50 化学 辐照 生物 DNA 基因 内分泌学 生物化学 转录因子 氧化应激 DNA结合蛋白 物理 核物理学
作者
Hamed Rezaeejam,Alireza Shirazi,Pantea Izadi,Javad Tavakkoly‐Bazzaz,Mahmoud Ghazi‐Khansari,Majid Valizadeh,Ghasem Azizi Tabesh
出处
期刊:Journal of Cancer Research and Therapeutics [BioMed Central]
卷期号:14 (12): 1070-1070 被引量:15
标识
DOI:10.4103/0973-1482.196758
摘要

Ionizing radiation is a critical threat to biomolecules, especially DNA. Various combinatorial compounds have been studied to protect this biomolecule. Melatonin has been reported as a direct and indirect free radical scavenger, but in this study, we explored the effect of melatonin on assisting in DNA repair by expression of Cdkn1a and Rad50; both of these genes are involved in DNA repair signaling, induced by radiation in rat peripheral blood.Rats were irradiated with single whole-body linear accelerator X-ray radiation doses of 2 and 8 Gy with or without melatonin (100 mg/kg body weight) pretreatments. The rats were randomly divided into nine groups and given an intraperitoneal injection of melatonin or the same volume of vehicle alone 1 h before radiation. Blood samples were taken 8, 24, and 48 h postradiation to measure gene expression of Cdkn1a and Rad50 using quantitative reverse transcription polymerase chain reaction technique.Melatonin pretreatment increased the expression of Cdkn1a and Rad50 in 8 and 24 h postradiations (2 and 8 Gy) (P < 0.05), and there was no significant difference in 48 h postradiation compared to the radiation-only and vehicle plus radiation (2 and 8 Gy) groups.Based on our results, pretreatment with melatonin (100 mg/kg) may ameliorates injurious effects of 2 and 8 Gy ionization radiation by increasing the expression level of Cdkn1a and Rad50 in rat peripheral blood and assist in DNA double-strand breaks repair, especially during the early postradiation.
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