A review on emerging principles and strategies for colorimetric and fluorescent detection of alkaline phosphatase activity

Alkaline phosphatase (ALP) is a natural enzyme that is able to catalyze the dephosphorylation of phosphate esters. It participates in a great number of biological processes ranging from various metabolisms to signal transduction and cellular regulation. Since the abnormality of ALP activity in body is closely associated with many diseases, it has become an important biomarker for clinical diagnosis and treatment. Besides, it is often utilized in enzyme-linked immunosorbent assays. Given these demands, in the last few years considerable interest has been focused on exploring new materials and methods for ALP activity detection. In this review, we first made a clear classification on the principles that could be used for ALP activity determination. After that, emerging colorimetric and fluorescent strategies designed on the basis of these principles were systematically summarized. Finally, some perspectives on ALP activity analysis were discussed, hoping to inspire future efforts in the field.