Vitellogenin receptor selectively endocytoses female-specific and highly-expressed hemolymph proteins in the silkworm,Bombyx mori

卵黄原蛋白 家蚕 血淋巴 卵黄发生 卵母细胞 生物 卵巢 九氟化硫 细胞生物学 卵子发生 受体 免疫荧光 家蚕 分子生物学 内科学 内分泌学 生物化学 胚胎 抗体 重组DNA 基因 夜蛾 免疫学 医学
作者
Chaoshan Han,Enxiang Chen,Guanwang Shen,Zhixin Peng,Yinying Xu,Haiyan Zhang,Hongling Liu,Yandi Zhang,Jinxin Wu,Ying Lin,Qingyou Xia
出处
期刊:Biochemistry and Cell Biology [Canadian Science Publishing]
卷期号:95 (4): 510-516 被引量:15
标识
DOI:10.1139/bcb-2016-0255
摘要

VgR, a member of the LDLR family, functions to transport vitellogenin into the ovaries to protome ovarian growth and embryonic development. In insects, the only widely accepted ligand of VgR is Vg. Recently, BmVgR has been shown to interact with BmSP1 in vitro. Therefore, in this study, we evaluated whether BmVgR could transport BmSP1 into certain cells. Although BmVgR could combine with BmVg and BmSP1, BmVgR did not affect the amount of BmSP1 taken up by Sf9 cells. Parallel immunofluorescence showed that most BmVg and BmVgR were localized in the inner oocyte membrane, showing tissue localization similar to that of BmVg labeled with pHrodo Red absorbed by the ovaries on day 2 of pupation. Although BmSP1 showed localization similar to BmVgR during the same phase, little BmSP1 was present in the ovary. Additionally, BmSP1 did not exist in ovaries when the ovaries contained BmVgR on day 5 of pupation, suggesting that BmSP1 in the ovaries was not endocytosed by BmVgR. In summary, BmVgR could facilitate uptake of BmVg by developing oocytes, but did not modulate in the transport of BmSP1.
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