Global warming could accelerate the spread of invasive species to higher latitudes and intensify their effects on native species. Here, we report results of two years of field surveys along a latitudinal gradient (21°N to 31°N) in southern China, to determine the species structure of the invasive plant Alternanthera philoxeroides community. We also performed a replacement series experiment (mono and mixed) to evaluate the effects of elevated temperature on the competitiveness of A. philoxeroides with the native co-occurring species Digitaria sanguinalis. In the field survey, we found that the dominance of A. philoxeroides increased with increasing of latitude gradient while cover of D. sanguinalis decreased. In monospecific plantings, artificial warming reduced the length of D. sanguinalis roots. In mixed plantings, warming reduced both A. philoxeroides abundance and D. sanguinalis stem length when A. philoxeroides was more prevalent in the planting. Warming also significantly reduced D. sanguinalis biomass, but increased that of A. philoxeroides. In addition, elevated temperatures significantly reduced the relative yield (RY) of D. sanguinalis, particularly when A. philoxeroides was planted in higher proportion in the plot. These results suggest that the invasiveness of A. philoxeroides increased with increasing latitude, and that warming may increase the effectiveness of its interspecific competition with D. sanguinalis. Hence, under global warming conditions, the harm to native species from A. philoxeroides would increase at higher latitudes. Our findings are critical for predicting the invasiveness of alien species under climate change.