侧流烟
化学
色谱法
香烟烟雾
检出限
气相色谱/串联质谱法
质谱法
气相色谱法
同位素稀释
气相色谱-质谱法
烟雾
有机化学
毒理
生物
作者
Gala Chapman,Juliana Giraldo Junco,Roberto Bravo Cardenas,Clifford H. Watson,Liza Valentín-Blasini
出处
期刊:Beiträge zur Tabakforschung international
日期:2019-12-01
卷期号:28 (7): 300-309
标识
DOI:10.2478/cttr-2019-0011
摘要
Summary Although 2-nitropropane is a potentially harmful compound present in cigarette smoke, there are few fully-validated, modern methods to quantitate it in mainstream cigarette smoke. We developed an isotope dilution gas chromatography-tandem mass spectrometry (ID-GC-MS/MS) method for the detection of 2-nitropropane in mainstream cigarette smoke. The vapor fraction of mainstream cigarette smoke was collected in inert polyvinyl fluoride gas sampling bags and extracted with hexanes containing isotopically labeled internal standard, then purified and concentrated via solid-phase extraction using a normal phase silica adsorbent and a 100% dichloromethane eluant. This method is sensitive enough to measure vapor phase 2-nitro-propane concentrations in the nanogram range, with a 19 ng per cigarette method limit of detection. Product variability estimated from the analysis of 15 cigarette products yielded relative standard deviations ranging from 5.4% to 15.7%, and estimates of precision from two quality control products yielded relative standard deviations of 9.49% and 14.9%. Under the Health Canada Intense smoking regimen, 2-nitropropane in machine-generated mainstream smoke from 15 cigarette products ranged from 98.3 to 363 ng per cigarette.
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