清脆的
生物
基因组工程
Cas9
合成生物学
CRISPR干扰
基因组编辑
多粘菌拟杆菌
计算生物学
转录激活物样效应核酸酶
核酸酶
反式激活crRNA
多路复用
代谢工程
基因
遗传学
细菌
作者
Christoph Schilling,Mattheos A. G. Koffas,Volker Sieber,Jochen Schmid
标识
DOI:10.1021/acssynbio.0c00424
摘要
Transcriptional perturbation using inactivated CRISPR-nucleases (dCas) is a common method in eukaryotic organisms. While rare examples of dCas9-based tools for prokaryotes have been described, multiplexing approaches are limited due to the used effector nuclease. For the first time, a dCas12a derived tool for the targeted activation and repression of genes was developed. Therefore, a previously described SoxS activator domain was linked to dCas12a to enable the programmable activation of gene expression. A proof of principle of transcriptional regulation was demonstrated on the basis of fluorescence reporter assays using the alternative host organism
科研通智能强力驱动
Strongly Powered by AbleSci AI