Xiang Sun,Lei Song,Wenjuan Yang,Lili Zhang,Meng Li,Xiaoshuang Li,Geng Tian,Weiwei Wang
出处
期刊:Methods in molecular biology日期:2020-01-01卷期号:: 13-32被引量:13
标识
DOI:10.1007/978-1-0716-0904-0_2
摘要
Nanopore sequencing is a method for determining the order and modifications of DNA/RNA nucleotides by detecting the electric current variations when DNA/RNA oligonucleotides pass through the nanometer-sized hole (nanopore). Nanopore-based DNA analysis techniques have been commercialized by Oxford Nanopore Technologies, NabSys, and Sequenom, and widely used in scientific researches recently including human genomics, cancer, metagenomics, plant sciences, etc., moreover, it also has potential applications in the field of healthcare due to its fast turn-around time, portable and real-time data analysis. Those features make it a promising technology for the point-of-care testing (POCT) and its potential clinical applications are briefly discussed in this chapter.