荧光
聚集诱导发射
动力学
生物物理学
蛋白质聚集
化学
放松(心理学)
纳米技术
材料科学
生物化学
生物
神经科学
物理
光学
量子力学
作者
Abeer Alghamdi,Li Hung C. Chung,Olaf J. Rolinski
出处
期刊:Methods in molecular biology
日期:2020-08-28
卷期号:: 167-177
标识
DOI:10.1007/978-1-0716-0928-6_11
摘要
Polypeptide assembly and aggregation are the common forms of its physiological and pathological activity, and monitoring them on a molecular level is critical for resolving numerous medical (e.g., onset of neurodegenerative diseases) or biological problems. Sensitivity of the intrinsic fluorescence of protein to its assembly, aggregation, or complexation offers a noninvasive methodology for identifying and determining different stages of these processes. In this protocol, we present the approach based on the time-resolved emission spectra (TRES), which reveals the number of fluorescent residues, the presence of dielectric relaxation, and the changes in fluorescence kinetics during aggregation.
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