嘧霉胺
心脏毒性
发育毒性
斑马鱼
生物
毒性
毒理
药理学
化学
环境化学
生态学
杀虫剂
内科学
遗传学
医学
基因
妊娠期
怀孕
作者
Yunlong Meng,Keyuan Zhong,Juhua Xiao,Yong Huang,You Wei,Lin Tang,Suping Chen,Juan Wu,Jinze Ma,Zigang Cao,Xinjun Liao,Huiqiang Lu
出处
期刊:Chemosphere
[Elsevier BV]
日期:2020-04-25
卷期号:255: 126889-126889
被引量:68
标识
DOI:10.1016/j.chemosphere.2020.126889
摘要
Pyrimethanil is a broad-spectrum fungicide commonly used in the prevention and treatment of Botrytis cinerea. However, little information is available in the literature to show the toxicity of Pyrimethanil to cardiac development. In this study, we used an experimental animal model to explore the developmental and cardiac toxicity of Pyrimethanil in aquatic vertebrates; we exposed zebrafish embryos to Pyrimethanil at concentrations of 2, 4, and 6 mg/L from 5.5 to 72 h post fertilisation. We found that Pyrimethanil caused a decrease in the hatching rate, heart rate, and survival rate of zebrafish embryos. Pyrimethanil exposure also resulted in pericardial and yolk sac edema, spinal deformity, and heart loop failure. Moreover, Pyrimethanil increased reactive oxygen stress levels and heightened the activity of superoxide dismutase and catalase. Alterations were induced in the transcription of apoptosis-related genes (p53, Bax, Bcl2, Casp 9, and Casp6l1) and heart development-related genes (Tbx2b, Gata4, Myh6, Vmhc, Nppa, Bmp2b, Bpm 4, and Bpm 10). Our data showed that the activation of Wnt signalling by BML-284 could partially rescue the malformed phenotype caused by Pyrimethanil. Our results provide new evidence for Pyrimethanil's toxicity and the danger of its residues in the environment and agricultural products.
科研通智能强力驱动
Strongly Powered by AbleSci AI