先天免疫系统
体内
免疫系统
骨髓
功能(生物学)
抗体
免疫学
细胞生物学
生物
化学
生物技术
作者
Gaël Boivin,Julien Faget,Pierre‐Benoit Ancey,Aspasia Gkasti,Julie Mussard,Camilla Engblom,Christina Pfirschke,Caroline Contat,Justine Pascual,Jessica Vázquez,Nathalie Bendriss‐Vermare,Christophe Caux,Marie‐Catherine Vozenin,Mikaël J. Pittet,Matthias Gunzer,Etienne Meylan
标识
DOI:10.1038/s41467-020-16596-9
摘要
Abstract Neutrophils are an essential part of the innate immune system. To study their importance, experimental studies often aim to deplete these cells, generally by injecting anti-Ly6G or anti-Gr1 antibodies. However, these approaches are only partially effective, transient or lack specificity. Here we report that neutrophils remaining after anti-Ly6G treatment are newly derived from the bone marrow, instead of depletion escapees. Mechanistically, newly generated, circulating neutrophils have lower Ly6G membrane expression, and consequently reduced targets for anti-Ly6G-mediated depletion. To overcome this limitation, we develop a double antibody-based depletion strategy that enhances neutrophil elimination by anti-Ly6G treatment. This approach achieves specific, durable and controlled reduction of neutrophils in vivo, and may be suitable for studying neutrophil function in experimental models.
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