Lipid profile of human synovial fluid following intra-articular ankle fracture

脚踝 滑液 断裂(地质) 关节面 医学 口腔正畸科 材料科学 解剖 病理 骨关节炎 复合材料 替代医学
作者
Elizabeth M. Leimer,Kirk L. Pappan,Dana L. Nettles,Richard D. Bell,Mark E. Easley,Steven A. Olson,Lori A. Setton,Samuel B. Adams
出处
期刊:Journal of Orthopaedic Research [Wiley]
卷期号:35 (3): 657-666 被引量:16
标识
DOI:10.1002/jor.23217
摘要

This study characterizes the metabolic profile of synovial fluid after intra-articular ankle fracture with an emphasis on changes in the lipid profile. Bilateral ankle synovial fluid from 19 patients with unilateral intra-articular ankle fracture was submitted for metabolic profiling. Contralateral ankle synovial fluid from each patient served as a matched control. Seven patients participated in a second bilateral synovial fluid collection after 6 months. Random forest classification, matched pairs t-tests (α < 0.01), repeated measures ANOVA with post-test contrasts (α < 0.01), correlation to cytokines and matrix metalloproteinases, and fracture and injury classification analyses yielded key lipid biomarkers in synovial fluid following intra-articular fracture. Free fatty acids, sphingomyelins, and lysolipids demonstrated significant elevation in fractured ankles at baseline. Fatty acids and sphingomyelins showed a significant decrease 6 months post-surgery. Random forest analysis showed predominantly fatty acids differentiating between groups. Significant correlations included fatty acids, sphingomyelins, and lysolipids with inflammatory cytokines and matrix metalloproteinases. Fracture classification showed increased fatty acids, lysolipids, and inositol metabolites as fracture severity increased. Fatty acid and sn-1 lysolipid elevation could be detrimental to the joint, as these strongly correlated with matrix metalloproteinases and TNF-α. This elevation also suggests involvement of phospholipase A2 , a potential target for therapeutic intervention. Together with elevated 2-hydroxyl fatty acids, these findings suggest elevated sn-1 lysolipids, sphingomyelins, and subsequent lipid metabolites in synovial fluid as biomarkers of ankle injury. Reversal of this signature after 6 months suggests temporary involvement of these metabolites in disease progression, although they may activate signaling pathways which drive progression to osteoarthritis. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:657-666, 2017.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
可乐发布了新的文献求助10
刚刚
哈哈哈完成签到,获得积分10
2秒前
2秒前
在水一方应助sunidea采纳,获得10
4秒前
共享精神应助激动的士萧采纳,获得10
6秒前
6秒前
Lucas应助冲冲冲冲冲冲采纳,获得10
6秒前
向雅发布了新的文献求助30
7秒前
9秒前
红红的红红发布了新的文献求助200
9秒前
young发布了新的文献求助20
10秒前
maomao发布了新的文献求助10
10秒前
STAR_To完成签到,获得积分10
10秒前
air-yi完成签到,获得积分10
10秒前
11秒前
不配.应助满穗采纳,获得20
13秒前
酷波er应助Ijaz采纳,获得10
13秒前
代骜珺发布了新的文献求助10
14秒前
14秒前
snail完成签到,获得积分10
14秒前
李健应助小龙女采纳,获得10
15秒前
不配.应助123采纳,获得10
15秒前
15秒前
15秒前
信江书院发布了新的文献求助10
15秒前
16秒前
liu完成签到,获得积分10
17秒前
keyanzhang完成签到 ,获得积分0
18秒前
liu发布了新的文献求助10
20秒前
20秒前
20秒前
瘦瘦含巧发布了新的文献求助10
21秒前
zsc完成签到,获得积分10
22秒前
丘比特应助可乐采纳,获得10
22秒前
23秒前
maomao完成签到,获得积分10
25秒前
冷艳的姿完成签到,获得积分10
25秒前
852应助书霂采纳,获得50
26秒前
小龙女发布了新的文献求助10
27秒前
27秒前
高分求助中
Sustainability in Tides Chemistry 2800
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
Rechtsphilosophie 1000
Bayesian Models of Cognition:Reverse Engineering the Mind 888
Le dégorgement réflexe des Acridiens 800
Defense against predation 800
Very-high-order BVD Schemes Using β-variable THINC Method 568
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3136127
求助须知:如何正确求助?哪些是违规求助? 2787029
关于积分的说明 7780244
捐赠科研通 2443154
什么是DOI,文献DOI怎么找? 1298899
科研通“疑难数据库(出版商)”最低求助积分说明 625294
版权声明 600870